Effect of glycerol and H3PO4 on the bioactivity and degradability of rod-like SBA-15 particles with active surface for bone tissue engineering applications

In the quest to increase quality of life and expectancy, there is a great demand develop advanced medical devices capable facing current health challenges. Santa Barbara Amorphous (SBA-15) mesoporous silica has been demonstrated be remarkable option for variety biomedical applications, from tissue engineering drug delivery, due its unique hierarchical porous structure. This work reports on effect using different ratios concentrations an acid mixture mediated by HCl/H3PO4 that in combination with glycerol-modified silane precursor favoured obtaining bioactive SBA-15 rods. The surfactant was removed solvent extraction which allowed keep substantial amount silanol groups (SiOH) at surface. All evaluated samples showed homogeneous rod shape particle size, as well high bioactivity after being immersed 14 days simulated body fluid (SBF). Different structural arrangements needle-type carbonate hydroxyapatite (HCA) crystals were produced upon immersion SBF. By scanning electron microscopy (SEM) characterization, it observed rod-shaped materials displayed higher degradability when concentration H3PO4 increased. An indirect cell viability assay MC3T3-E1 osteoblast cells performed incubating culture medium exposed particles 1 4 days. results obtained WST-8 fluorescent staining no negative viability, indicating lack cytotoxicity rod-like particles.